vazyme one step cloning kit

  • Frontiers EgmiR5179 Regulates Lipid Metabolism by

    Jul 26 2021  The CDS of EgMADS16 was cloned using different primers and ligated into pGreen II 0800 miRNA LUC pGADT7 and pGBKT7 using a One Step Cloning Kit Vazyme according to the principle of homologous recombination.

  • C113 ClonExpress MultiS One Step Cloning Kit

    ClonExpress MultiS One Step Cloning Kit is anew version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help ofth iskit upto fivefragments

  • VazymeGeneron

    Vazyme. Vazyme offers a range of products for molecular biology et more specially for applications like PCR qPCR RT PCR and Next Generation Sequencing NGS . Vazyme products are recognized worldwide and can be found in numerous publications in renowned magazines such as Nature Cell or New England Medicine Cell research. 2015 25 .

  • ProductCatalogue

    One Step RT PCR 50rxn 50µl/rxn P611 01 HiScriptIIOneStepRT PCRKit DyePlus HiScriptIIOneStepRT PCRKit 50rxn 50µl/rxn P612 01 Web vazyme Email global vazyme VazymeBiotech .Ltd. 3 DL2000PlusDNAMarker 250µl/500µl MD101 01/02 DL5000DNAMarker 250µl/500µl MD102 01/02 DL15000DNAMarker 250µl/500µl MD103 01/02

  • 2 Vazyme LAmp Master Mix Dye Plus BioLinksLabSupplies

    The protective agents included guarantees the stability of the activity of this Master Mix. The PCR product containing dA at 3 end can be cloned into T vector and is suitable for One Step Express cloning kit C112/C113 . Brand Vazyme Biotech.

  • C115 02 ClonExpress Ultra One Step Cloning Kit Biotrend

    Brand Vazyme. CloudFront. You might also be interested by the following products Cat# Description. Size. Price Excl. VAT. EM101 01. EasyPure Plasmid MiniPrep Kit. 50rxns . EM101 02. ClonExpress II One Step Cloning Kit. 50rxn . C113 01. ClonExpress MultiS One Step Cloning Kit

  • Clonexpress Ii One Step Cloning KitIκBα

    Allele In One Mouse Tail Direct Pcr System Allele In One Mouse Tail Direct Pcr Kit Igm Is One Of The First Types Of Antibodies All In One Pcr Mastermix Clonexpress Ii One Step Cloning Ki Clonexpress Ii One Step Cloning Kit Clonexpress Ii One Step Cloning Kit Cells Direct One Step Qrt Pcr Kit Accurate Chemicals One Step Cell Direct One

  • TA Cloning Kits Thermo Fisher ScientificUS

    TA Cloning technology greatly simplifies traditional restriction and ligation cloning with a one step cloning strategy that eliminates the need for any enzymatic modifications of the PCR product and not require the use of primers that contain restriction enzyme sites. TA cloning relies on the complementary bases of adenine A and thymine T on different DNA fragments to hybridize together.

  • NEBuilder HiFi DNA Assembly Cloning Kit NEB

    NEBuilder HiFi DNA Assembly Cloning Kit was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments regardless of fragment length or end compatibility. This method has been used to assemble either single stranded oligonucleotides or different sizes of DNA fragments with varied overlaps 15–30 bp .

  • ClonExpress II One Step Cloning Kit #C112

    ClonExpress II One Step Cloning Vazyme #C112 is a simple fast and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly the vector is linearized at the cloning

  • Molecular BiologyAfirmus Biosource

    For Molecular Research Vazyme offers various reagents for Molecular Cloning Mutagenesis Nucleic Acid Electrophoresis Reverse Transcription qPCR Master Mix and genome editing Popular products HiScript II U One Step qRT PCR Probe Kit VAHTS Universal DNA Library Prep Kit for Illumina 2 x Phanta Max Master Mix Dye Plus

  • Nanjing Vazyme Medical TechnologyFibroblast like

    The Nanjing Vazyme Medical Technology reagent is RUO Research Use Only to test human serum or cell culture lab samples. To purchase these products for the MSDS Data Sheet protocol storage conditions/temperature or for the concentration please contact Vazyme. Other Nanjing products are available in stock.

  • HighFidelityPCR FastCloningLubioScience

    pCE Zero Vector Linearized is supplied withClonExpress Ultra One Step Cloning Kit Vazyme #C115 . It is highly recommended to use Vazyme s APP CEDesign for easy primer design. Single fragments and multiple fragments were cloned or assembled to pCE Zero Vector usingClonExpress Ultra One Step Cloning Kit Vazyme #C115 respectively.

  • ClonExpress Ultra One Step Cloning KitVazyme Biotech

    ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Co. Ltd ClonExpress Ultra One Step Cloning Kit is a new generation of recombinant cloning kit which can quickly and efficiently clone 1 5 fragments to any position of any vector. The highly optimized 2 ClonExpress Mix 50℃ reaction 5 15 Min completes the reorganization and the operation is simple and quick.

  • Vazyme Biotech Co. Ltd

    HiScript II U One Step qRT PCR Probe Kit Q222 CN Raw Materials for 2019 nCoV Nucleic Acid Detection.HiScript II U One Step qRT PCR Probe Kit Vazyme #Q222 CN is specially designed for qPCRs that directly use RNA i.e. virus RNA as templates.

  • Vazyme Biotechcloning kits enzymesLubioScience

    Vazyme Biotech offers a broad range of general molecular biology and NGS reagents and kits. These include one step cloning kits Trizol based RNA isolation reagent various enzymes and a broad range of Illumina compatible RNA and DNA library prep kits. Try Vazyme s VAHTS beads for NGS library purificationa drop in replacement for AMPure XP

  • P211 P212 2X Taq Plus Master Mix

    obtained PCR products are compatible with ClonExpress II One Step Cloning Kit series Vazyme #C112 #C113 . The PCR products contain A at the 3’ end and can be directly cloned into T Vectors. One unit U is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid insoluble products in 30 min at 74 ℃with

  • The Royal Society of Chemistry

    ClonExpress II One Step Cloning Kit Vazyme Biotech Co. Ltd . Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and

  • Frontiers BEL1 like Homeodomain Protein BLH6a Is a

    Jun 25 2021  About 11 PCR fragments were cloned into the pGADT7 vector through the recombination method by using the ClonExpressII One Step Cloning Kit Vazyme Biotech Co. Ltd. Nanjing Jiangsu China . The full lengths of coding regions cloned in pGADT7 were sequence confirmed. The coding regions of 14 TFs were synthesized and cloned into pGADT7.

  • Vazyme Clinisciences

    Vazyme. Vazyme offers a range of products for molecular biology et more specially for applications like PCR qPCR RT PCR and Next Generation Sequencing NGS . Vazyme products are recognized worldwide and can be found in numerous publications in renowned magazines such as Nature Cell or New England Medicine Cell research. 2015 25 .

  • Functional dissection and modulation of the BirA protein

    Jul 21 2021  Finally the linear pMTLcas vector and the sgRNA HA1 fragment were assembled in one step using the ClonExpress MultiS One Step Cloning Kit Vazyme Biotech Nanjing China yielding the pMTLcas birA plasmid for the deletion of birA in C. ljungdahlii.

  • ClonExpress II One Step Cloning Kitgenebiosystems

    Cloning Gel and Isolation. Cloning and Gel. Cloning. TA cloning Kits Blunt end Cloning Kits Universal Cloning Kits Competent Cells DNA Ligases Mutagenesis Kits Protein Expression In Vitro Transcription Gel Electrophoresis. Agarose DNA Gel Stain and Loading Buffer DNA Ladders Low MW Range DNA Ladders Mid MW Range DNA Ladders High

  • ClonExpress II One Step Cloning Kitvazyme

    ClonExpress II One Step Cloning Kit ClonExpress IIOneStepCloningKit 50bp 10kbDNAPCR >95

  • HiScript II One Step RT PCR Kit Vazyme Biotech Co. Ltd

    HiScript II One Step RT PCR Kit Vazyme Biotech Co. Ltd HiScript II One Step RT PCR Kit is specially designed for end point PCR detection using RNA as template such as RNA virus . Using gene specific primers GSP reverse transcription and PCR reactions are completed in one tube no additional tube opening/pipetting operations are required which greatly improves detection throughput and

  • Universal Cloning KitsGeneBio Sys your Molecular and

    ClonExpress MultiS One Step Cloning Kit . Vazyme ClonExpress One Step Cloning is a simple fast and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly the vector is linearized at the cloning site.

  • C112 02 ClonExpress II One Step Cloning Kit Biotrend

    Diagnostic. Custom production. CRO services. Protocols. Special offer. Contact. Reagents and instruments for immunology cell biology and molecular biology. Biotrend >. > > ClonExpress II One Step Cloning Kit.

  • C115 ClonExpress UItra One Step Cloning Kit

    VazymeBiotechCo. Ltd Order global vazyme Support global vazyme High throughput Cloning SeamlessAssembly Site specificMutagenesis 5Applications PCRtemplates primers linearizedvectors. C115 ClonExpress UItra One Step Cloning Kit

  • Vazyme KyvoBio

    Vazyme offers a range of products for molecular biology et more specially for applications like PCR qPCR RT PCR and Next Generation Sequencing NGS . Vazyme products are recognized worldwide and can be found in numerous publications in renowned magazines such as Nature Cell or

  • Pmd19 T Simple TaKaRa Bioz

    The three PCR fragments were mixed with pMD19 T vector Takara Japan and ligated using ClonExpress MultiS One Step Cloning Kit Vazyme China according to the manufacturer’s protocol. .. The resulting products were transformed into E . coli DH5α and transformants were selected on LB agar containing 50 μg/mL Kan. PCRs were performed on

  • ClonExpressIIOneStepCloningkitCellagen Tech

    amplificationwithahigh fidelitypolymerases i.e.PhantaMaxSuper FidelityDNAPolymerase Vazyme #P505 arehighlyrecommended. Cloning Site pU C18 A L ine arized by do uble dig estion HndIII EcoRI B amH I C Lin earized by PC Ramplification Restrictionsiteswillbe fullyremained during cloning

  • VazymeStratech

    ClonExpress II One Step Cloning Kit uses a highly efficient homologous recombination technology to clone DNA products to very high efficiency in a simple rapid one step procedure. Any amplified product s can be cloned into to any linearized vector at any site. The kit uses Exnase II a unique recombinant enhancer which can significantly improve the efficiency of recombination 100 cfu ng

  • Fast Cloning Tamar Laboratory Supplies LTD.

    The insert and the linearized vector with overlapped sequences of 15 bp20 bp on both 5’ and 3’ end respectively are mixed in an appropriate ratio and incubated with recombinase Exnase at 50℃ for 515 min.The Vazyme ClonExpress UItra One Step Cloning kit is a novel cloning kit applicable for homologous recombination with 1

  • Rapid Cloning Product Vazyme Biotech Co. Ltd

    Rapid Cloning Vazyme Biotech Co. Ltd. ClonExpress Ultra One Step Cloning Kit is a new generation of recombinant cloning kit which can quickly and efficiently clone 1

  • In Fusion HD Cloning Plus Kits for seamless DNA cloning

    In Fusion HD Cloning Plus also includes In Fusion HD Enzyme Premix. In Fusion HD enzyme mix fuses DNA fragments efficiently and precisely by recognizing a 15 bp overlap at their ends without the use of ligase. Provided in a liquid format as a 5X enzyme premix in 20 μl or 100 μl aliquots depending on kit

  • Molecular BiologyAbsource Diagnostics

    ClonExpress MultiS One Step Cloning Kit. ClonExpress II One Step Cloning Kit. 2 Vazyme LAmp Master Mix an enzyme containing 3′ >5′ exonuclease activity. Green Taq Mix. 5min TA/Blunt Zero Cloning Kit. 5min TM TA/Blunt Zero Cloning Kit is a second generation TOPO cloning kit that contains a second generation Topoisomerase a vector

  • ftp.polgen

    One Step Cloning Technology. ClonExpress One Step Cloning Technology is a simple fast and highly efficient cloning kit which is based on homologous recombination technology. It allows to directly clone any amplified product s to any linearized vector at any site. Firstly the expression vector will be linearized at the cloning site of choice.